EDUCATION:

Ph.D.      Physics, 2003, Kumaun University, Nainital, INDIA.

M. Sc.  Laser and molecular spectroscopy (Physics), 1996, Kumaun University,          Nainital, INDIA

M. Sc. Physics (Electronics), 1993, Kumaun University, Nainital, INDIA

B. Sc.  Physics, Chemistry, Mathematics, 1991, Kumaun University, INDIA

Ph.D. Thesis Supervisor:      Prof. H. B. Tripathi    

Ph.D. Thesis Title:     “Photoinduced Electronic Excited State Relaxation and Proton Transfer Phenomena in some Hydrogen Bonded Molecules”

AWARDS AND HONORS:

·                Young Scientist Project Awarded under DST (India) Fast Track Scheme. 

·                “Outstanding Presentation Award” in INSAT-98 conference held in IISc, Bangalore, organized by IAIA India.

·                Awarded various National/ State level Scholarships.

Present occupation:          

Lecturer, Department of Physics, MMV-Banaras Hindu University, Varanasi-221005, India

Principle Investigator, (Under Department of Science and Technology, New Delhi, Young Scientist Fast Track Scheme, Project cost Rs. 20,50,000/=).

Title of the Project:

“Electronic structure and dynamics of photoinduced excited states in some hydrogen bonded molecular system and its applications in organized assemblies and nano-structured materials”.

PROFESSIONAL EXPERIENCE:

1.             Junior Research Fellow, March to April 1994, at Laser Raman Laboratory, Department of Physics, Indian Institute of Technology, (IIT) Kanpur, India.

2.             Research Assistant, 16^th October 1995 to 15^th April 1998 in the DST Project, at Photophysics Laboratory, Department of Physics, Kumaun University, Nainital, India.

3.             Senior Research Fellow,  16^th April 1998 to 30^th April 2003, CSIR, New Delhi India at Photophysics Laboratory,  Department of Physics,  Kumaun University, Nainital India.

4.             Research Associate: Photophysics Laboratory, Department of Physics, Kumaun University, Nainital, India. Project sponsored by Council of Scientific and Industrial Research, (CSIR) New Delhi, India since 1^st May 2003 to 30^th May 2006.

5.             Young Scientist: in Molecular Biophysics Unit, Indian Institute of Science, Bangalore from 1^st June 2006 to 30^th Feb 2008.

FIELD OF SPECIALIZATION: Ultra-fast Time Domain Fluorescence Spectroscopy and Microscopy, Laser and Molecular spectroscopy, Electronics and photonics, Electronic structure calculations through computation.

PROFESSIONAL SKILLS (TEACHING/RESEARCH EXPERIENCE):

1.             Three year teaching experience of Electronics and Modern Physics at Undergraduate classes and Laser & Molecular spectroscopy and Quantum Mechanics in Post Graduate classes in Department of Physics, Kumaon University Nainital. India and Banaras Hindu University, Varanasi, India

2.             Fabrication of electronic circuits like amplifier, oscillator, power supply, timer, transmitter / receiver, ms flash lamp etc. Design, maintenance and operation of spectrophotometer and spectrofluorometer for steady state and time domain measurements.

3.             I have studied the various primary photochemical processes viz. Photo-induced excited state proton transfer, electron transfer, charge transfer, energy transfer, solvent relaxation etc in some hydrogen bonded molecular systems.

4.             Basic knowledge of Fluorescence  in situ hybridization (FISH),  Fluorescence Recovery After Photo-bleaching (FRAP), Fluorescence Resonance Energy Transfer Microscopy (FRETM), Fluorescence Lifetime Imaging Microscope (FLIM), Optical Tweezers,  X-ray crystallography, NMR spectroscopy, CD, FTIR.

5.             Experience in maintenance and operation of various types of fluorescence spectroscopic instruments viz. Spectrophotometer (ratio recording Backman DK-A, JASCO V-550), Spectrofluorometer (JASCO-FP-777, ISA JOBIN YVON-SPEX-FL3-22), Time domain spectrophotometer Edinburg FLA 199) with short pulse generator like micro-second, nano-second and pico second range pulse through flash lamp, (H2 and N2 gas discharge lamp) / mode locked, frequency doubled Nd-YAG laser coupled with cavity dumped dye laser, Nitrogen laser coupled with BOXCAR (SRS-245), time domain confocal fluorescence Microscope, Pico-Quaunt (Nicon fluoroscence microscope coupled with TCSPC system and pico second diode laser ) and low temperature instruments like cryostat APD – E-CS-202) respectively.

6.             Sample purification, crystallization and preparation of plastic films viz. protic, aprotic and ion-exchange by cast method. Preparation of various glass systems doped with organic and inorganic ions and chelet formation.  DNA, protein isolation, purification and characterization.

7.             Data acquisition and analysis of Steady State and Time domain results through related software for fluorescence (GRAMA/386 of Galactic), Origin 6.0 and absorption data analysis as well as important Word Processing & Mathematical data analysis. Besides this different software used for theoretical computational calculations viz. Gaussian 98, Gaussian 03, Gauss view, Molden for electronic structure exploration of molecular system.

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SEMINAR / Symposia / Conferences:

·                National Symposium on Molecular Spectroscopy & Lasers, BHU, Varanasi in 1994.

·                Workshop on Advanced Laser Spectroscopy, I.I.T. Kanpur, 1995.

·                International Conference on Spectroscopy, BARC, Mumbai, Jan. 1996.

·                International Discussion Meeting on "Ultra Fast Chemical Phenomena". IISc Banglore, March 2-6, 1998.

·                XIII ISAS National Symposium on Analytical Techniques 2001; INSAT-98; Nov. 24th to 25th  in Indian Institute of Science Bangalore Under the title "Spectral Anomalies in Computer Based Spectrofluorometer" Oral Presentation.

·                ANACON -98, National conferences organized by IASI at Jawaharlal Science Center, Mumbai. "Spectral Anomalies in Computer Based Spectrofluorometer" Oral presentation.

·                Trombay symposium on Radiation and Photochemistry (TSRP-2000) held in BARC, Jan. 12-17th 2000. Under title “ An Optical approach of sensing water”

·                National laser symposium held in LASTEC, New Delhi since 13-15th December 2000. Under title "Study of Photophysics and lasing action of 3-hydroxyflavon in polymeric media".

·                Structure and dynamics of complex chemical systems held in IISc Bangalore since 9-13 January 2001. Under title “Photophysics of 3-hydroxy 2- naptholic acid”.

·                India-Japan workshop on new Advance Materials in Molecular Electronics (NAMME), held at NPL-New-Delhi, India10-11 December 2001, “Fluorescence studies of salicylic acid.

·                Third Asian Photochemistry Conference (APC-2002) held at Hotel Centaur, Juhu Beach, Mumbai, India Jan. 6 –11 2002. PP 86, under title “Photoinduced proton transfer in 1-hydroxy 2-naphthoic acid: experimental and theoretical investigations.

·                "XIX International conference on photochemistry" 1st to 6th August 1999, Duke University, Durhan N.C. 27708 USA. Organized by Prof. J. D. Simon, under title "Photophysics and photochemistry of 7-azaindole and 7-hydroxy quinoline in polymeric system" pp 125 and "Photoindused proton translocation in salicylic acid (an experimental and Theoretical investigation)" pp 126. By H. Mishra, H. C. Joshi and H.B. Tripathi. *

·                "National Laser symposium" Dec. 15-17, 1999, School of Physics, University of Hyderabad, Under title "Study of excitation energy transfer between acriflavin and rhodhamine 6G in nafion film as a pH sensor". pp 213 by H. Mishra.

·                "Spectroscopy of Lanthanides and Actinides SLA - 99" Nov. 16-19, 1999, BARC Mumbai - 400085. Under title "Dye sensitized rare earth doped polymer as a luminescence solar collector" pp 246. By H. Mishra.

·                Methods and applications of fluorescence MAF, Prague, held on dated August 24-27, 2003 under title “Edge excitation red shift and energy migration in quinine bisulphate dication” poster presentation number 1 by H. Mishra, T.C. Pant and H. B. Tripathi.*

·                National Laser symposium on Cellular and Molecular Bio-physics, held at NIMHANS, Banglore January 14^th –17^th 2004.

·                XXX Optical society of India (OSI) Symposium on Optics and Optoelectronics (SOOP-05), to be held on NPL New Delhi Jan 19-21 2005

·                National Symposium on “Recent Trends in Fluorescence Spectroscopy and its Applications” Held in Department of Physics, Kumaon University Nainital. Dec. 1-3^rd 2006. (Oral Presentation)

·                Trombay Symposium on Radiation and Photochemistry (TSRP-2006) held in BARC, Jan. 5-9^th 2006.

·                1^st Asian Conference on Spectroscopy and Bio-spectroscopy", held at IISc, Bangalore India during January 29^th to 3rd February, 2007.

·                Invited talk in workshop “Computers in Chemistry” held in SSJ Campus Almora Kumoun University Nainital, during 21 to 23^rd Nov 2007.

·                Trombay Symposium on Radiation and Photochemistry (TSRP-2008) held in YASHADA, Pune Jan. 7th-11^th  2008.

·                Invited talk in workshop “Recent advance in Solar Energy Materials” held in Jurahat Campus Srinagar, HNB Garwal University to be held during 21 to 23^rd March 2008.

*     (abstract accepted and published in book, but could not attend the conference)

SERC School \ Short Course:

·         SERC School on "Ultrafast Studies" School of Physics, University of Hyderabad, India. July 10-29, 2006.

·         SERC School on "Bio-Photonics" Centre for Advance Technology (CAT) Indore, India. Feb 6-21, 2006.

·         Short course on “Principles and Applications of Time Resolved Fluorescence Spectroscopy” organized by Prof. J. R. Lakowicz at Centre of Fluorescence spectroscopy, University of Maryland USA. March 22^nd to 26^nd March 2004.

·         SERC school on “Precision Physical Measurement and Measurement science” held in National Physical Laboratory (NPL), New Delhi, India. December 4^th to 23^rd 2000.

·         SERC School on “Electronic Structure and Physics of Materials” held in Satyendra Nath Bose National Center for Basic Sciences, (SNBNCBS) Calcutta, India October 31st to November 19th, 1999.

·         SERC school on "Non Linear Optics and Laser Spectroscopy" Indian Institute of Technology (IIT) New Delhi, India. March 10-28, 1997.

MEMBERSHIP OF PROFESSINAL SOCIETIES:       

1.       Life member of Indian laser association (ILA –541).

2.             Who’s Who in the Fluorescence world (Kluwer Academic/Plenum Publisher)

3.             Laser Focus World and Photonics

4.             “PAHAR” Uttranchal magazine.

5.             Life member of  ISRAP

Supervised:  M. Sc. Dissertation of Mr. Ahsok Kumar Yadav and (2) Mr. Vikash Babu, Department of Biotechnology, K.U. Nainital India.

·        Past Research and Achievements

I did my Post doctoral and Ph.D. with Prof. H. B. Tripathi at the Photophysics Laboratory, Department of Physics, D.S.B. Campus, Kumaon University Nainital India in the field of ultra fast time resolved fluorescence spectroscopy of intra and inter molecular hydrogen bonded molecular system under different environmental conditions with special emphasis on the of excited state proton transfer (ESPT) and its applications. Hydrogen bonding plays a very important role for existing of life in the universe. Excited state intramolecular proton transfer, is one of the most important and characteristic relaxation route in the hydrogen bonded molecular systems.  This work was carried out during the tenure of research assistantship under the DST project “Facilities for the Peco-second spectroscopy”. Then I was awarded Senior Research Fellowship by CSIR, New Delhi, India. Besides this I had also studied role of diffusion on Förster resonance energy transfer (FRET) and its application as pH sensor with the Prof. T.C. Pant, Charge transfer studies were carried out with Prof. D.D. Pant, Emeritus Professor in the Department.  Theoretical part of the research work was carried out at IIT Kanpur, India with Prof. N. Sathyamurthy. Further, I was awarded Young Scientist project by DST India and I run this project  in Molecular Biophysics Unit, Indian Institute of Science, Bangalore, under the supervision of Prof. P. Balaram in the area of “Photoinducied dynamics of a glycolytic enzyme triosephosphate isomerase (TIM) isolated from Plasmodium falciparum”.

                         Moreover, during the course of experimental investigation it was found that the spectra obtained by advance technology based spectrofluorometer JASCO FP-777 showed many anomalies in fluorescence excitation and emission spectra which could not be removed by the manufacturer. I, thus, started a comprehensive program to remove these anomalies in the spectrofluorometer and obtained a correction coefficient data for spectral measurement for this spctrofluoremeter. (Mishra et. al. Review of Scientific Instrumentation, in press)

References:

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